Journal: Cancer Microenvironment

Article Title: Mammographic Density and Matrix Metalloproteinases in Breast Tissue

doi: 10.1007/s12307-009-0031-x

Figure Lengend Snippet: Proportion of subjects with positive stains for MMPs and TIMP3 by ethnicity

Article Snippet: Following TMA construction, 5 μm sections of TMA blocks were sent to Wake Forest University for immunohistochemical staining with the following markers: MMP1 (1906-1), MMP3 (1908-1), MMP9 (1939-1), MMP12 (1906-1; all Epitomics, Inc., Burlingame, CA), and TIMP3 (CA 0577; Cell Applications, Inc., San Diego, CA).

Techniques:

Journal: Cancer Microenvironment

Article Title: Mammographic Density and Matrix Metalloproteinases in Breast Tissue

doi: 10.1007/s12307-009-0031-x

Figure Lengend Snippet: Percent density for MMP1, MMP12, and TIMP3 by tissue type and ethnicity (Adjusted for age at mammogram, BMI, ethnicity, parity, age at first live birth, age at menarche, menopausal status, hormone use at mammogram, family history of breast cancer, and tumor stage)

Article Snippet: Following TMA construction, 5 μm sections of TMA blocks were sent to Wake Forest University for immunohistochemical staining with the following markers: MMP1 (1906-1), MMP3 (1908-1), MMP9 (1939-1), MMP12 (1906-1; all Epitomics, Inc., Burlingame, CA), and TIMP3 (CA 0577; Cell Applications, Inc., San Diego, CA).

Techniques:

Journal: Diagnostic Pathology

Article Title: Promoter methylation and expression of TIMP3 gene in gastric cancer

doi: 10.1186/1746-1596-8-110

Figure Lengend Snippet: Gastric carcinoma TIMP3 promoter methylation and protein expression

Article Snippet: TIMP3 antibody and SP kit were purchased from Boster Biological Engineering Co., Ltd. (Dalian).

Techniques: Methylation, Expressing

Journal: Diagnostic Pathology

Article Title: Promoter methylation and expression of TIMP3 gene in gastric cancer

doi: 10.1186/1746-1596-8-110

Figure Lengend Snippet: TIMP3 protein immunohistochemisty (SP 400×): a, normal gastric tissue; b, early gastric cancer; c, advanced gastric cancer; d, transfer of lymph node.

Article Snippet: TIMP3 antibody and SP kit were purchased from Boster Biological Engineering Co., Ltd. (Dalian).

Techniques:

Journal: Diagnostic Pathology

Article Title: Promoter methylation and expression of TIMP3 gene in gastric cancer

doi: 10.1186/1746-1596-8-110

Figure Lengend Snippet: Relationship between advanced gastric cancer pathology TIMP3 methylation and its protein expression level

Article Snippet: TIMP3 antibody and SP kit were purchased from Boster Biological Engineering Co., Ltd. (Dalian).

Techniques: Methylation, Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The common DEGs of four gene expression profiles (adj. P value <0.05, |logFC|> 2.0).

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Gene Expression

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: PPI network and hub genes' identification. (a) PPI network was structured by the 54 upregulated DEGs using STRING database. (b) The top 10 hub genes in the PPI network were screened ground on their connectivity degree. The genes such as CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA are represented from red (high degree) to yellow (low degree). (c) Gene Ontology (GO) chord diagram of the top genes in GBM.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Top ten hub genes with higher degree of connectivity.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Binding Assay

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The AUC analysis of hub genes. AUC analysis of CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA for distinguishing GBM samples from normal tissues.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: OS, DFS, and univariate and multivariate Cox analysis of the hub genes overexpressed in GBM patients. (a) OS of the hub genes overexpressed in GBM sufferers. (b) DFS of the hub genes overexpressed in GBM sufferers. (c) Univariate and multivariate Cox analysis of TIMP1 expression and other clinical pathological factors for OS. HR, hazard ratio.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation between immune infiltrates and TIMP1 expression in GBM. (a) TIMP1 expression was definitely connected with CD4+ T cell, B cell, T cell regulatory (Tregs), neutrophils, cancer-associated fibroblast, macrophage M1, and myeloid dendritic cell. (b–d) Higher infiltration of Tregs, cancer-associated fibroblast, and mast cell correlated with worse prognosis.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation analysis between TIMP1 and related genes and markers of immune cells in TIMER.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Downregulation of TIMP1 inhibits cell proliferation, migration, and invasion in U-87 MG and U-118 MG cells. (a, b) The results of qPCR and WB showed that compared with many glioblastoma cell lines, U-87 MG and U-118 MG cell lines were selected for TIMP1 gene related function test. (c, d) qPCR and WB showed that TIMP1 expression was inhibited in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (e, f) CCK-8 and colony formation assays revealed that the proliferation abilities were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (g, h) Transwell assay revealed that cell migration and invasion were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Migration, Expressing, Transfection, CCK-8 Assay, Transwell Assay

Journal: Ecotoxicology and environmental safety

Article Title: Endosulfan promotes cell proliferation and extracellular matrix accumulation through TGF-β/Smad signaling pathway in HRMCs.

doi: 10.1016/j.ecoenv.2021.113040

Figure Lengend Snippet: Fig. 2. Dose-effects of endosulfan on ECM accumulation related genes in HRMCs. HRMCs were exposed to different concentrations of endosulfan or D control for 48 h. Relative mRNA expression levels of MMP2 (A) and TIMP3 (B) were measured by qRT-PCR. Representative Western blots was shown (C) and protein expression levels of LN, TIMP3 and MMP3 were densitometrically quantified (D). *P < 0.05, **P < 0.01 vs D.

Article Snippet: Primary antibodies include MMP3 (Cat. AF513, R&D Systems, Shanghai, China), LN (Cat. sc-13144, Santa Cruz), Smad2/3 (Cat. E1A6362, Cat. E1A6449, EnoGene, Nanjing, China) and p-Smad2/3(p-Ser467/425) (Cat. AM12299, Cat. AM1398 AURAGENE BIOTECH, Hunan, China), Smad7 (Cat. 25840-1-AP, Proteintech, Wuhan, China), TIMP3 (Cat. A00477, BOSTER), IL-8 (Cat. A2541, Abclonal, Wuhan, China), p21 (Cat. A1483, Abclonal, Wuhan, China), p27 (Cat. A0290, Abclonal, Wuhan, China), MMP2 (Cat. A6247, Abclonal, Wuhan, China).

Techniques: Control, Expressing, Quantitative RT-PCR, Western Blot